Methods Silica gel column chromatography and HPLC were used to isolate and purify the compounds and NMR, ESI-MS, EI-MS, and circular dichroism spectrum was applied to elucidate their structures. 方法用硅胶柱色谱、高效液相色谱、NMR、ESI-MS、EI-MS和圆二色光谱等各种波谱和光谱方法对该植物醋酸乙酯萃取部位的化学成分进行分离鉴定。
Analyzing secondary structure of β_2 microglobulin in Escherichia coli with circular dichroism spectrum 大肠杆菌表达的重组猪β2微球蛋白二级结构的圆二色谱分析
The effect of crystallization of hydroxyapatite on the secondary structure of bovine serum albumin ( BSA) was studied by circular dichroism spectrum, Fourier transform infrared spectroscopy, derivative, deconvolution and curve-fitting techniques in the present paper. 通过圆二色谱(CD)及傅里叶变换红外光谱及退卷积、曲线拟合等技术研究了羟基磷灰石结晶对牛血清白蛋白二级结构的影响。
In the presence of Cl-, the fluorescence spectrum and circular dichroism spectrum of EGX do not change but the thermostability of the enzyme improves. 离子的存在对EGX荧光光谱和圆二色性谱不产生明显影响,但能大大提高EGX的热稳定性。
Effects of Lanthanum(ⅲ) Ions on Circular Dichroism Spectrum of Yeast tRNA 丙氨酸(Ala.)镧离子对酵母tRNA圆二色谱的影响
The binding of Hypocrellin A ( HA) to DNA prior to and after irradiation and its effects on the fluorescence of DNA-EB complex and changes of temperature-dependent UV absorption and circular dichroism spectrum of DNA were studied in this work. 本文研究了光敏氧化前后甲素与DNA的结合以及这种结合对DNA-EB复合物荧光强度、DNA融解温度和圆二色谱的影响。
Circular dichroism ( CD) spectrum analysis showed that the percentage of α-helix,β-sheet and random coil of day lily tubulin is 27.24%, 24.48% and 48.28%, respectively, indicating a typical feature of globulin. 此时的最大发射峰为338nm,圆二色光谱分析二级结构表明α-螺旋占27.24%,β-折叠占24.48%,无规卷曲为48.28%,呈典型球蛋白特征。
It was demonstrated that the enzyme has 28.8% a-helix, 27.9% p-sheet, 43.2% random coil by the analysis of circular dichroism spectrum. 圆二色谱(CD)对其二级结构的分析显示,蔗糖酶分子中α-螺旋占28.8%,β-折叠占27.9%,无规则卷曲占43.2%。
Prokaryotic Expression and Purification of Mammalian Cell-entry Proteins 4 E ( mce4 E) of Mycobacterium bovis and Analysis of Circular Dichroism Spectrum 牛分枝杆菌哺乳动物细胞侵袭蛋白4E的原核表达、纯化及其圆二色谱分析
The secondary structure of the protein was determined by circular dichroism ( CD) spectrum. The a-Helix, P-Turn and Random were 47.9%, 23.1% and 29.0%. 应用CD光谱检测蛋白质的二级结构,α-螺旋、β-转角和任意卷曲分别占47.9%,23.1%,29.0%。